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smart cdna library construction kit  (TaKaRa)


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    Structured Review

    TaKaRa smart cdna library construction kit
    Smart Cdna Library Construction Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3011 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+library+construction+kit/SMART+cDNA+Library+Construction+Kit/us12617869-108-20-25
    Average 96 stars, based on 3011 article reviews
    smart cdna library construction kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    cDNA Library Assay:

    Article Title: The GhNAC75-GhMAPK7-3 module positively regulates drought tolerance of Gossypium hirsutum
    Article Snippet: .. Screening of a yeast one-hybrid (Y1H) cDNA library Total RNA was obtained from leaves from three-week-old TM-1 seedlings, and a Y1H cDNA library was created by a cDNA library construction kit provided by Clontech. ..

    Article Title: Bannakunin: a dual-target Kunitz inhibitor bridging anticoagulation (FXa/XIIa) and anti-platelet (α2β1/P2Y12) pathways
    Article Snippet: .. The 5′ sense degenerate primer (5′-TACTATGG(A/C) GGTTG(T/C) G(A/G) GGG(A/C) AA-3′) was designed based on the conserved signature of the black fly Kunitz family, while the 3′ PCR primer (5′ -CGGGGTACGATGAGACACCA-3′) was provided by Takara’s cDNA library construction kit (Japan). ..

    Article Title: Bannakunin: a dual-target Kunitz inhibitor bridging anticoagulation (FXa/XIIa) and anti-platelet (α2β1/P2Y12) pathways.
    Article Snippet: .. The 5′ sense degenerate primer (5′- T A C T A T G G(A/C) GGTTG(T/C) G(A/G) GGG(A/C) AA-3′) was designed based on the conserved signature of the black fly Kunitz family, while the 3′ PCR primer (5′ - C G G G G T A C G A T G A G A C A C C A-3′) was provided by Takara’s cDNA library construction kit (Japan). ..

    Article Title: Sibakazin: a novel black fly derived non-classical kazal protease inhibitor, targeting antiplatelet (P2Y12/GPVI) pathways without perturbing coagulation.
    Article Snippet: While Kazal-type inhibitors from hematophagous animals are well-known anticoagulants, their roles in platelet regulation remain largely unexplored.. Here, we report the characterization of Sibakazin, a single-domain Kazaltype inhibitor from the salivary glands of the blood-sucking black fly Simulium bannaense.. Structurally, Sibakazin possesses a non-canonical acidic N-terminus and methionine at the P1 site.

    Article Title: OsMADS55 nuclear translocation enhances OsATL15-mediated thiamethoxam uptake in rice for controlling the brown planthopper.
    Article Snippet: .. The complementary DNA (cDNA) from O. sativa was ligated into the SfiI-SfiI sites of the yeast prey vector pPR3-NubG (Dualsystems Biotech, Schlieren, Switzerland), using the cDNA Library Construction Kit (TaKaRa, Dalian, Japan) to establish the prey library. .. To screen for physical interactors of OsATL15 using the yeast split-ubiquitin high-throughput system, the Saccharomyces cerevisiae reporter strain NMY51 (MATa, his3Δ200, trp1-901, leu2-3, 112, ade2, LYS2:: (lexAop)4-HIS3, ura3::(lexAop)8-lacZ, (lexAop)8-ADE2, GAL4; Dualsystems Biotech) containing the OsATL15-Cub bait construct was transformed with 25 μg of the cDNA library prey plasmid.

    Article Title: GhAGL80–9 positively regulates flowering by activating the expression of GhAP1-D3 and GhAGL9 in Gossypium hirsutum L.
    Article Snippet: .. Screening of yeast one-hybrid (Y1H) cDNA library Total RNA was obtained from leaves of TM-1 plants (3 TLS), and a Y1H cDNA library was created by a cDNA library construction kit (Clontech). ..

    Article Title: Comparative Transcriptomic Analysis of Male and Female Gonads in Hemibagrus guttatus (Lacepède)
    Article Snippet: .. RNA-seq libraries were prepared and sequenced at Zhenyue Biotechnology Laboratory (Wuhan, China). cDNA libraries were prepared using the cDNA Library Construction Kit (TaKaRa Biotechnology (Dalian) Co., Ltd., Dalian, China), with an initial total RNA amount ranging from 2.14 to 3.25 μg. ..

    Article Title: Rubber tree iron superoxide dismutase gene HbFSD1 is positively regulated by the basic leucine zipper transcription factor HbbZIP37 and enhances drought tolerance in transgenic Arabidopsis.
    Article Snippet: Rubber tree (Hevea brasiliensis) is an economically important tropical tree species that produces natural rubber (cis-1,4-polyisoprene).. Climate change leads to an increasing frequency of drought, posing significant challenges to natural rubber production.. Superoxide dismutase (SOD) is a crucial enzyme that plays a vital role in the response to abiotic stresses in plants, but its function in the rubber tree is still unclear.

    Polymerase Chain Reaction:

    Article Title: Bannakunin: a dual-target Kunitz inhibitor bridging anticoagulation (FXa/XIIa) and anti-platelet (α2β1/P2Y12) pathways
    Article Snippet: .. The 5′ sense degenerate primer (5′-TACTATGG(A/C) GGTTG(T/C) G(A/G) GGG(A/C) AA-3′) was designed based on the conserved signature of the black fly Kunitz family, while the 3′ PCR primer (5′ -CGGGGTACGATGAGACACCA-3′) was provided by Takara’s cDNA library construction kit (Japan). ..

    Article Title: Bannakunin: a dual-target Kunitz inhibitor bridging anticoagulation (FXa/XIIa) and anti-platelet (α2β1/P2Y12) pathways.
    Article Snippet: .. The 5′ sense degenerate primer (5′- T A C T A T G G(A/C) GGTTG(T/C) G(A/G) GGG(A/C) AA-3′) was designed based on the conserved signature of the black fly Kunitz family, while the 3′ PCR primer (5′ - C G G G G T A C G A T G A G A C A C C A-3′) was provided by Takara’s cDNA library construction kit (Japan). ..

    Article Title: Sibakazin: a novel black fly derived non-classical kazal protease inhibitor, targeting antiplatelet (P2Y12/GPVI) pathways without perturbing coagulation.
    Article Snippet: While Kazal-type inhibitors from hematophagous animals are well-known anticoagulants, their roles in platelet regulation remain largely unexplored.. Here, we report the characterization of Sibakazin, a single-domain Kazaltype inhibitor from the salivary glands of the blood-sucking black fly Simulium bannaense.. Structurally, Sibakazin possesses a non-canonical acidic N-terminus and methionine at the P1 site.

    Plasmid Preparation:

    Article Title: OsMADS55 nuclear translocation enhances OsATL15-mediated thiamethoxam uptake in rice for controlling the brown planthopper.
    Article Snippet: .. The complementary DNA (cDNA) from O. sativa was ligated into the SfiI-SfiI sites of the yeast prey vector pPR3-NubG (Dualsystems Biotech, Schlieren, Switzerland), using the cDNA Library Construction Kit (TaKaRa, Dalian, Japan) to establish the prey library. .. To screen for physical interactors of OsATL15 using the yeast split-ubiquitin high-throughput system, the Saccharomyces cerevisiae reporter strain NMY51 (MATa, his3Δ200, trp1-901, leu2-3, 112, ade2, LYS2:: (lexAop)4-HIS3, ura3::(lexAop)8-lacZ, (lexAop)8-ADE2, GAL4; Dualsystems Biotech) containing the OsATL15-Cub bait construct was transformed with 25 μg of the cDNA library prey plasmid.

    Article Title: Rubber tree iron superoxide dismutase gene HbFSD1 is positively regulated by the basic leucine zipper transcription factor HbbZIP37 and enhances drought tolerance in transgenic Arabidopsis.
    Article Snippet: Rubber tree (Hevea brasiliensis) is an economically important tropical tree species that produces natural rubber (cis-1,4-polyisoprene).. Climate change leads to an increasing frequency of drought, posing significant challenges to natural rubber production.. Superoxide dismutase (SOD) is a crucial enzyme that plays a vital role in the response to abiotic stresses in plants, but its function in the rubber tree is still unclear.

    RNA Sequencing:

    Article Title: Comparative Transcriptomic Analysis of Male and Female Gonads in Hemibagrus guttatus (Lacepède)
    Article Snippet: .. RNA-seq libraries were prepared and sequenced at Zhenyue Biotechnology Laboratory (Wuhan, China). cDNA libraries were prepared using the cDNA Library Construction Kit (TaKaRa Biotechnology (Dalian) Co., Ltd., Dalian, China), with an initial total RNA amount ranging from 2.14 to 3.25 μg. ..

    Construct:

    Article Title: Rubber tree iron superoxide dismutase gene HbFSD1 is positively regulated by the basic leucine zipper transcription factor HbbZIP37 and enhances drought tolerance in transgenic Arabidopsis.
    Article Snippet: Rubber tree (Hevea brasiliensis) is an economically important tropical tree species that produces natural rubber (cis-1,4-polyisoprene).. Climate change leads to an increasing frequency of drought, posing significant challenges to natural rubber production.. Superoxide dismutase (SOD) is a crucial enzyme that plays a vital role in the response to abiotic stresses in plants, but its function in the rubber tree is still unclear.

    Recombinant:

    Article Title: Rubber tree iron superoxide dismutase gene HbFSD1 is positively regulated by the basic leucine zipper transcription factor HbbZIP37 and enhances drought tolerance in transgenic Arabidopsis.
    Article Snippet: Rubber tree (Hevea brasiliensis) is an economically important tropical tree species that produces natural rubber (cis-1,4-polyisoprene).. Climate change leads to an increasing frequency of drought, posing significant challenges to natural rubber production.. Superoxide dismutase (SOD) is a crucial enzyme that plays a vital role in the response to abiotic stresses in plants, but its function in the rubber tree is still unclear.

    Incubation:

    Article Title: Rubber tree iron superoxide dismutase gene HbFSD1 is positively regulated by the basic leucine zipper transcription factor HbbZIP37 and enhances drought tolerance in transgenic Arabidopsis.
    Article Snippet: Rubber tree (Hevea brasiliensis) is an economically important tropical tree species that produces natural rubber (cis-1,4-polyisoprene).. Climate change leads to an increasing frequency of drought, posing significant challenges to natural rubber production.. Superoxide dismutase (SOD) is a crucial enzyme that plays a vital role in the response to abiotic stresses in plants, but its function in the rubber tree is still unclear.



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